› 2hx9

X-ray diffraction
1.7Å resolution

Crystal structure of Cu(I) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM", at pH4

Released:
DOI: 10.2210/pdb2hx9/pdb
PDB entry 2hx9 coloured by chain, front view.
PDB entry 2hx9 coloured by chain, side view.
PDB entry 2hx9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Engineering copper sites in proteins: loops confer native structures and properties to chimeric cupredoxins.
Li C, Banfield MJ, Dennison C
J Am Chem Soc 129 709-18 (2007)
PMID: 17227035

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 13.79 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 99%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU1 2 x CU1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 38.42Å b: 65.335Å c: 97.242Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.218
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
2 more