› 2i21

X-ray diffraction
1.84Å resolution

Bacteriorhodopsin/lipid complex, T46V mutant

Released:
DOI: 10.2210/pdb2i21/pdb
PDB entry 2i21 coloured by chain, front view.
PDB entry 2i21 coloured by chain, side view.
PDB entry 2i21 coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Propagating structural perturbation inside bacteriorhodopsin: crystal structures of the M state and the D96A and T46V mutants.
Lanyi JK, Schobert B
Biochemistry 45 12003-10 (2006)
PMID: 17002299

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 26.93 KDa
Source organism: Halobacterium salinarum
Expression system: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-262; Coverage: 95%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand RET 1 x RET
Ligand LI1 13 x LI1
Ligand SQU 1 x SQU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P63
Unit cell:
a: 61.069Å b: 61.069Å c: 110.534Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.195 0.26
Expression system: Halobacterium salinarum

Quick links

Citations

4 review citations

Bacteriorhodopsin: Would the real structural intermediates please stand up?
Wickstrand et al. (2015)
3 more

3 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
2 more