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A comparison of stigmatellin conformations, free and bound to the photosynthetic reaction center and the cytochrome bc1 complex.

Lancaster CR, Hunte C, Kelley J, Trumpower BL, Ditchfield R
J Mol Biol 368 197-208 (2007)
Cited: 31 times
EuropePMC logo PMID: 17337272

Abstract

We describe in detail the conformations of the inhibitor stigmatellin in its free form and bound to the ubiquinone-reducing (Q(B)) site of the reaction center and to the ubiquinol-oxidizing (Q(o)) site of the cytochrome bc(1) complex. We present here the first structures of a stereochemically correct stigmatellin in complexes with a bacterial reaction center and the yeast cytochrome bc1 complex. The conformations of the inhibitor bound to the two enzymes are not the same. We focus on the orientations of the stigmatellin side-chain relative to the chromone head group, and on the interaction of the stigmatellin side-chain with these membrane protein complexes. The different conformations of stigmatellin found illustrate the structural variability of the Q sites, which are affected by the same inhibitor. The free rotation about the chi1 dihedral angle is an essential factor for allowing stigmatellin to bind in both the reaction center and the cytochrome bc1 pocket.

Reviews - 2ibz mentioned but not cited (3)

  1. Structural analysis of cytochrome bc1 complexes: implications to the mechanism of function. Xia D, Esser L, Tang WK, Zhou F, Zhou Y, Yu L, Yu CA. Biochim Biophys Acta 1827 1278-1294 (2013)
  2. Structural basis of resistance to anti-cytochrome bc₁ complex inhibitors: implication for drug improvement. Esser L, Yu CA, Xia D. Curr Pharm Des 20 704-724 (2014)
  3. Quinone binding sites of cyt bc complexes analysed by X-ray crystallography and cryogenic electron microscopy. Kao WC, Hunte C. Biochem Soc Trans 50 877-893 (2022)

Articles - 2ibz mentioned but not cited (6)



Reviews citing this publication (5)

  1. Catalytic Reactions and Energy Conservation in the Cytochrome bc1 and b6f Complexes of Energy-Transducing Membranes. Sarewicz M, Pintscher S, Pietras R, Borek A, Bujnowicz Ł, Hanke G, Cramer WA, Finazzi G, Osyczka A. Chem Rev 121 2020-2108 (2021)
  2. The nonheme iron in photosystem II. Müh F, Zouni A. Photosynth Res 116 295-314 (2013)
  3. Protein conformational changes involved in the cytochrome bc1 complex catalytic cycle. Cooley JW. Biochim Biophys Acta 1827 1340-1345 (2013)
  4. Design and use of photoactive ruthenium complexes to study electron transfer within cytochrome bc1 and from cytochrome bc1 to cytochrome c. Millett F, Havens J, Rajagukguk S, Durham B. Biochim Biophys Acta 1827 1309-1319 (2013)
  5. Rieske iron-sulfur protein of the cytochrome bc(1) complex: a potential target for fungicide discovery. Yang WC, Li H, Wang F, Zhu XL, Yang GF. Chembiochem 13 1542-1551 (2012)

Articles citing this publication (17)



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