› 2idq

X-ray diffraction
0.9Å resolution

Structure of M98A mutant of amicyanin, Cu(II)

Released:
DOI: 10.2210/pdb2idq/pdb
PDB entry 2idq coloured by chain, front view.
PDB entry 2idq coloured by chain, side view.
PDB entry 2idq coloured by chain, top view.
Source organism: Paracoccus denitrificans
Primary publication:
Generation of novel copper sites by mutation of the axial ligand of amicyanin. Atomic resolution structures and spectroscopic properties.
Carrell CJ, Ma JK, Antholine WE, Hosler JP, Mathews FS, Davidson VL
Biochemistry 46 1900-12 (2007)
PMID: 17295442

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-540135 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amicyanin Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.45 KDa
Source organism: Paracoccus denitrificans
Expression system: Escherichia coli
UniProt:
  • Canonical: P22364 (Residues: 27-131; Coverage: 100%)
Gene names: ami, mauC
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 1 x CU
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 28.57Å b: 56.16Å c: 26.97Å
α: 90° β: 96.63° γ: 90°
R-values:
R R work R free
0.109 not available 0.13
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Cupredoxins--a study of how proteins may evolve to use metals for bioenergetic processes.
Choi et al. (2011)
2 more

5 mentions without citation

3D-SURFER: software for high-throughput protein surface comparison and analysis.
La et al. (2009)
4 more