› 2ift

X-ray diffraction
2.3Å resolution

Crystal structure of putative methylase HI0767 from Haemophilus influenzae. NESG target IR102.

Released:
DOI: 10.2210/pdb2ift/pdb
PDB entry 2ift coloured by chain, front view.
PDB entry 2ift coloured by chain, side view.
PDB entry 2ift coloured by chain, top view.
Source organism: Haemophilus influenzae
Entry authors: Vorobiev SM, Su M, Seetharaman J, Shastry R, Janjua H, Cunningham K, Ma LC, Xiao R, Liu J, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(966) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(966) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155220 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase D Chains: A, B
Molecule details ›
Chains: A, B
Length: 201 amino acids
Theoretical weight: 23.19 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P44869 (Residues: 1-193; Coverage: 100%)
Gene names: HI_0767, rsmD
Sequence domains: Conserved hypothetical protein 95
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 71.337Å b: 57.873Å c: 47.522Å
α: 90° β: 107.36° γ: 90°
R-values:
R R work R free
0.223 0.223 0.266
Expression system: Escherichia coli

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