› 2igm

X-ray diffraction
1.9Å resolution

Crystal structure of recombinant pyranose 2-oxidase H548N mutant

Released:
DOI: 10.2210/pdb2igm/pdb
PDB entry 2igm coloured by chain, front view.
PDB entry 2igm coloured by chain, side view.
PDB entry 2igm coloured by chain, top view.
Source organism: Trametes ochracea
Primary publication:
Structural basis for substrate binding and regioselective oxidation of monosaccharides at C3 by pyranose 2-oxidase.
Kujawa M, Ebner H, Leitner C, Hallberg BM, Prongjit M, Sucharitakul J, Ludwig R, Rudsander U, Peterbauer C, Chaiyen P, Haltrich D, Divne C
J Biol Chem 281 35104-15 (2006)
PMID: 16984920

Function and Biology Details

Reaction catalysed: 
D-glucose + O(2) = 2-dehydro-D-glucose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182318 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyranose 2-oxidase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 623 amino acids
Theoretical weight: 69.39 KDa
Source organism: Trametes ochracea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7ZA32 (Residues: 1-623; Coverage: 100%)
Gene name: p2o
Sequence domains: GMC oxidoreductase
Structure domains: Phage tail proteins - 2 layer sandwich fold

Ligands and Environments


Cofactor: Ligand FAD 8 x FAD
1 bound ligand:
Ligand MES 8 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 168.431Å b: 103.142Å c: 168.915Å
α: 90° β: 106.08° γ: 90°
R-values:
R R work R free
0.152 0.152 0.189
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Flavoenzymes.
Joosten et al. (2007)
4 more