› 2igt

X-ray diffraction
1.89Å resolution

Crystal Structure of the SAM Dependent Methyltransferase from Agrobacterium tumefaciens

Released:
DOI: 10.2210/pdb2igt/pdb
PDB entry 2igt coloured by chain, front view.
PDB entry 2igt coloured by chain, side view.
PDB entry 2igt coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Entry authors: Kim Y, Joachimiak A, Xu X, Gu J, Edwards A, Savchenko A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-108938 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine-dependent methyltransferase domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 332 amino acids
Theoretical weight: 37.43 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A9CKD6 (Residues: 1-309; Coverage: 100%)
Gene name: Atu0340
Sequence domains: S-adenosylmethionine-dependent methyltransferase
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SAM 3 x SAM
Ligand GOL 2 x GOL
Ligand ACY 6 x ACY
Ligand FMT 7 x FMT
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 83.941Å b: 145.555Å c: 204.895Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.192
Expression system: Escherichia coli BL21(DE3)

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