› 2ihx

Solution NMR

Solution Structure of the Rous Sarcoma Virus Nucleocapsid Protein:uPsi RNA Packaging Signal Complex

Released:
DOI: 10.2210/pdb2ihx/pdb
PDB entry 2ihx coloured by chain, ensemble of 20 models, front view.
PDB entry 2ihx coloured by chain, ensemble of 20 models, side view.
PDB entry 2ihx coloured by chain, ensemble of 20 models, top view.
Source organism: Rous sarcoma virus
Primary publication:
Solution structure of the Rous sarcoma virus nucleocapsid protein: muPsi RNA packaging signal complex.
Zhou J, Bean RL, Vogt VM, Summers M
J Mol Biol 365 453-67 (2007)
PMID: 17070546

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136797 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleocapsid protein p12 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.71 KDa
Source organism: Rous sarcoma virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03322 (Residues: 503-563; Coverage: 9%)
Gene name: gag
Sequence domains: Zinc knuckle
Structure domains: Zinc finger, CCHC-type
uPsi RNA Chain: B
Molecule details ›
Chain: B
Length: 75 nucleotides
Theoretical weight: 24.27 KDa
Source organism: Rous sarcoma virus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: 1H-1H distance restraints, Hydrogen-bond restraints, Torsion angle restraints, Inter-phosphate restraints
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

The structural biology of HIV assembly.
Ganser-Pornillos et al. (2008)
6 more

8 mentions without citation

Features, processing states, and heterologous protein interactions in the modulation of the retroviral nucleocapsid protein function.
Mirambeau et al. (2010)
7 more