› 2ijh

X-ray diffraction
1.8Å resolution

Crystal structure analysis of ColE1 ROM mutant F14W

Released:
DOI: 10.2210/pdb2ijh/pdb
PDB entry 2ijh coloured by chain, front view.
PDB entry 2ijh coloured by chain, side view.
PDB entry 2ijh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
New crystal structures of ColE1 Rom and variants resulting from mutation of a surface exposed residue: Implications for RNA-recognition.
Struble EB, Ladner JE, Brabazon DM, Marino JP
Proteins 72 761-8 (2008)
PMID: 18260113

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-527215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein rop Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 63 amino acids
Theoretical weight: 7.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03051 (Residues: 1-63; Coverage: 100%)
Gene name: rop
Sequence domains: Rop protein
Structure domains: Helix Hairpins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 102.48Å b: 44.79Å c: 45.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Influenza virus non-structural protein NS1: interferon antagonism and beyond.
Marc D. (2014)
4 other articles

1 mention without citation

Evaluation of underutilized Ethiopian potato (Plectranthus edulis (Vatke) Agnew) accessions in Oromia regional state, central Ethiopia.
Abdena et al. (2023)