› 2iuk

X-ray diffraction
2.4Å resolution

Crystal structure of Soybean Lipoxygenase-D

Released:
DOI: 10.2210/pdb2iuk/pdb
PDB entry 2iuk coloured by chain, front view.
PDB entry 2iuk coloured by chain, side view.
PDB entry 2iuk coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Crystal structures of vegetative soybean lipoxygenase VLX-B and VLX-D, and comparisons with seed isoforms LOX-1 and LOX-3.
Youn B, Sellhorn GE, Mirchel RJ, Gaffney BJ, Grimes HD, Kang C
Proteins 65 1008-20 (2006)
PMID: 17022084

Function and Biology Details

Reaction catalysed: 
Linoleate + O(2) = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-540672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seed linoleate 9S-lipoxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 864 amino acids
Theoretical weight: 96.87 KDa
Source organism: Glycine max
Expression system: Escherichia coli
UniProt:
  • Canonical: P24095 (Residues: 1-864; Coverage: 100%)
Gene names: LOX1.4, SC514
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 92.76Å b: 115.1Å c: 120.22Å
α: 90° β: 112.34° γ: 90°
R-values:
R R work R free
0.203 0.203 not available
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Evolutionary aspects of lipoxygenases and genetic diversity of human leukotriene signaling.
Horn et al. (2015)
3 more

6 mentions without citation

The structural basis for specificity in lipoxygenase catalysis.
Newcomer et al. (2015)
5 more