2iv3 › Citations

Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4.

Martinez-Fleites C, Proctor M, Roberts S, Bolam DN, Gilbert HJ, Davies GJ
Chem Biol 13 1143-52 (2006)
Related entries: 2iuy, 2iv7, 2iw1

Cited: 76 times
EuropePMC logo PMID: 17113996

Abstract

Glycosyltransferases (GTs) catalyze the synthesis of the myriad glycoconjugates that are central to life. One of the largest families is GT4, which contains several enzymes of therapeutic significance, exemplified by WaaG and AviGT4. WaaG catalyses a key step in lipopolysaccharide synthesis, while AviGT4, produced by Streptomyces viridochromogenes, contributes to the synthesis of the antibiotic avilamycin A. Here we present the crystal structure of both WaaG and AviGT4. The two enzymes contain two "Rossmann-like" (beta/alpha/beta) domains characteristic of the GT-B fold. Both recognition of the donor substrate and the catalytic machinery is similar to other retaining GTs that display the GT-B fold. Structural information is discussed with respect to the evolution of GTs and the therapeutic significance of the two enzymes.

Reviews - 2iv3 mentioned but not cited (1)

  1. Chapter 2: Biogenesis of the cell wall and other glycoconjugates of Mycobacterium tuberculosis. Kaur D, Guerin ME, Skovierová H, Brennan PJ, Jackson M. Adv Appl Microbiol 69 23-78 (2009)

Articles - 2iv3 mentioned but not cited (1)



Reviews citing this publication (11)

  1. Glycosyltransferases: structures, functions, and mechanisms. Lairson LL, Henrissat B, Davies GJ, Withers SG. Annu Rev Biochem 77 521-555 (2008)
  2. Natural-product sugar biosynthesis and enzymatic glycodiversification. Thibodeaux CJ, Melançon CE, Liu HW. Angew Chem Int Ed Engl 47 9814-9859 (2008)
  3. Recent structures, evolution and mechanisms of glycosyltransferases. Breton C, Fournel-Gigleux S, Palcic MM. Curr Opin Struct Biol 22 540-549 (2012)
  4. Glycosyltransferases: mechanisms and applications in natural product development. Liang DM, Liu JH, Wu H, Wang BB, Zhu HJ, Qiao JJ. Chem Soc Rev 44 8350-8374 (2015)
  5. The impact of enzyme engineering upon natural product glycodiversification. Williams GJ, Gantt RW, Thorson JS. Curr Opin Chem Biol 12 556-564 (2008)
  6. Structure-function relationships of membrane-associated GT-B glycosyltransferases. Albesa-Jové D, Giganti D, Jackson M, Alzari PM, Guerin ME. Glycobiology 24 108-124 (2014)
  7. Carbohydrate synthesis by disaccharide phosphorylases: reactions, catalytic mechanisms and application in the glycosciences. Luley-Goedl C, Nidetzky B. Biotechnol J 5 1324-1338 (2010)
  8. Structures and mechanisms of the mycothiol biosynthetic enzymes. Fan F, Vetting MW, Frantom PA, Blanchard JS. Curr Opin Chem Biol 13 451-459 (2009)
  9. Glycosyltransferases, glycoside hydrolases: surprise, surprise! Henrissat B, Sulzenbacher G, Bourne Y. Curr Opin Struct Biol 18 527-533 (2008)
  10. Exploring genomes for glycosyltransferases. Hansen SF, Bettler E, Rinnan A, Engelsen SB, Breton C. Mol Biosyst 6 1773-1781 (2010)
  11. Mechanistic differences among retaining disaccharide phosphorylases: insights from kinetic analysis of active site mutants of sucrose phosphorylase and alpha,alpha-trehalose phosphorylase. Goedl C, Schwarz A, Mueller M, Brecker L, Nidetzky B. Carbohydr Res 343 2032-2040 (2008)

Articles citing this publication (63)



Quick links