› 2izl

X-ray diffraction
1.48Å resolution

STREPTAVIDIN-2-IMINOBIOTIN PH 7.3 I222 COMPLEX

Released:
DOI: 10.2210/pdb2izl/pdb
PDB entry 2izl coloured by chain, front view.
PDB entry 2izl coloured by chain, side view.
PDB entry 2izl coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Binding of biotin to streptavidin stabilizes intersubunit salt bridges between Asp61 and His87 at low pH.
Katz BA
J Mol Biol 274 776-800 (1997)
PMID: 9405158

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: B, D
Molecule details ›
Chains: B, D
Length: 123 amino acids
Theoretical weight: 12.97 KDa
Source organism: Streptomyces avidinii
UniProt:
  • Canonical: P22629 (Residues: 37-159; Coverage: 77%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand IMI 2 x IMI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: I222
Unit cell:
a: 95.41Å b: 106.54Å c: 48.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.211

Quick links

Citations

2 review citations

Pseudoreceptor models in drug design: bridging ligand- and receptor-based virtual screening.
Tanrikulu et al. (2008)
1 more

2 mentions without citation

Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site.
Korndörfer et al. (2002)
1 more