› 2j05

X-ray diffraction
1.5Å resolution

Crystal structure of the RasGAP SH3 domain at 1.5 Angstrom resolution

Released:
DOI: 10.2210/pdb2j05/pdb
PDB entry 2j05 coloured by chain, front view.
PDB entry 2j05 coloured by chain, side view.
PDB entry 2j05 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High resolution crystal structures of the p120 RasGAP SH3 domain.
Ross B, Kristensen O, Favre D, Walicki J, Kastrup JS, Widmann C, Gajhede M
Biochem Biophys Res Commun 353 463-8 (2007)
PMID: 17188236

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-539613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras GTPase-activating protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 65 amino acids
Theoretical weight: 7.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20936 (Residues: 281-341; Coverage: 6%)
Gene names: GAP, RASA, RASA1
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P61
Unit cell:
a: 32.8Å b: 32.8Å c: 183.33Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.177 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

p120-Ras GTPase activating protein (RasGAP): a multi-interacting protein in downstream signaling.
Pamonsinlapatham et al. (2009)
4 other articles

6 mentions without citation

Functional cross-talk between ras and rho pathways: a Ras-specific GTPase-activating protein (p120RasGAP) competitively inhibits the RhoGAP activity of deleted in liver cancer (DLC) tumor suppressor by masking the catalytic arginine finger.
Jaiswal et al. (2014)
5 more