› 2j4d

X-ray diffraction
1.9Å resolution

Cryptochrome 3 from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb2j4d/pdb
PDB entry 2j4d coloured by chain, front view.
PDB entry 2j4d coloured by chain, side view.
PDB entry 2j4d coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Cryptochrome 3 from Arabidopsis thaliana: structural and functional analysis of its complex with a folate light antenna.
Klar T, Pokorny R, Moldt J, Batschauer A, Essen LO
J Mol Biol 366 954-64 (2007)
PMID: 17188299

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183055 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cryptochrome DASH, chloroplastic/mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 525 amino acids
Theoretical weight: 60.33 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q84KJ5 (Residues: 45-569; Coverage: 92%)
Gene names: At5g24850, CRY3, CRYD, F6A4.60
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 2 x FAD
Ligand MHF 2 x MHF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 76.298Å b: 116.782Å c: 135.024Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.221
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The cryptochromes: blue light photoreceptors in plants and animals.
Chaves et al. (2011)
11 more

4 mentions without citation

Recognition and repair of UV lesions in loop structures of duplex DNA by DASH-type cryptochrome.
Pokorny et al. (2008)
3 more