› 2j7c

X-ray diffraction
2.09Å resolution

Beta-glucosidase from Thermotoga maritima in complex with phenylaminomethyl-derived glucoimidazole

Released:
DOI: 10.2210/pdb2j7c/pdb
PDB entry 2j7c coloured by chain, front view.
PDB entry 2j7c coloured by chain, side view.
PDB entry 2j7c coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Glycosidase inhibition: an assessment of the binding of 18 putative transition-state mimics.
Gloster TM, Meloncelli P, Stick RV, Zechel D, Vasella A, Davies GJ
J Am Chem Soc 129 2345-54 (2007)
PMID: 17279749

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-555165 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 53.94 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q08638 (Residues: 2-446; Coverage: 100%)
Gene name: bglA
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:
Ligand IDE 2 x IDE
Ligand ACT 1 x ACT
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 94.302Å b: 94.518Å c: 113.805Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.254
Expression system: Escherichia coli BL21

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Citations

13 review citations

β-Glucosidases.
Ketudat Cairns et al. (2010)
12 more