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Variable oligomerization modes in coronavirus non-structural protein 9.

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Ponnusamy R, Moll R, Weimar T, Mesters JR, Hilgenfeld R
OpenAccess logo J Mol Biol 383 1081-96 (2008)
Cited: 39 times
EuropePMC logo PMID: 18694760

Abstract

Non-structural protein 9 (Nsp9) of coronaviruses is believed to bind single-stranded RNA in the viral replication complex. The crystal structure of Nsp9 of human coronavirus (HCoV) 229E reveals a novel disulfide-linked homodimer, which is very different from the previously reported Nsp9 dimer of SARS coronavirus. In contrast, the structure of the Cys69Ala mutant of HCoV-229E Nsp9 shows the same dimer organization as the SARS-CoV protein. In the crystal, the wild-type HCoV-229E protein forms a trimer of dimers, whereas the mutant and SARS-CoV Nsp9 are organized in rod-like polymers. Chemical cross-linking suggests similar modes of aggregation in solution. In zone-interference gel electrophoresis assays and surface plasmon resonance experiments, the HCoV-229E wild-type protein is found to bind oligonucleotides with relatively high affinity, whereas binding by the Cys69Ala and Cys69Ser mutants is observed only for the longest oligonucleotides. The corresponding mutations in SARS-CoV Nsp9 do not hamper nucleic acid binding. From the crystal structures, a model for single-stranded RNA binding by Nsp9 is deduced. We propose that both forms of the Nsp9 dimer are biologically relevant; the occurrence of the disulfide-bonded form may be correlated with oxidative stress induced in the host cell by the viral infection.

Articles - 2j98 mentioned but not cited (3)

  1. Variable oligomerization modes in coronavirus non-structural protein 9. Ponnusamy R, Moll R, Weimar T, Mesters JR, Hilgenfeld R. J Mol Biol 383 1081-1096 (2008)
  2. Structural basis for dimerization and RNA binding of avian infectious bronchitis virus nsp9. Hu T, Chen C, Li H, Dou Y, Zhou M, Lu D, Zong Q, Li Y, Yang C, Zhong Z, Singh N, Hu H, Zhang R, Yang H, Su D. Protein Sci 26 1037-1048 (2017)
  3. A novel computational strategy for defining the minimal protein molecular surface representation. Grassmann G, Miotto M, Di Rienzo L, Gosti G, Ruocco G, Milanetti E. PLoS One 17 e0266004 (2022)


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