› 2j9i

Electron Microscopy
17Å resolution

Lengsin is a survivor of an ancient family of class I glutamine synthetases in eukaryotes that has undergone evolutionary re- engineering for a tissue-specific role in the vertebrate eye lens.

Released:
DOI: 10.2210/pdb2j9i/pdb
PDB entry 2j9i coloured by chain, front view.
PDB entry 2j9i coloured by chain, side view.
PDB entry 2j9i coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Lengsin is a survivor of an ancient family of class I glutamine synthetases re-engineered by evolution for a role in the vertebrate lens.
Wyatt K, White HE, Wang L, Bateman OA, Slingsby C, Orlova EV, Wistow G
Structure 14 1823-34 (2006)
PMID: 17161372
Related structures: EMD-1290

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lengsin Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 421 amino acids
Theoretical weight: 39.59 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8CIX8 (Residues: 128-218, 219-255, 256-328, 329-378, 379-454, 455-523, 524-563; Coverage: 73%)
Gene names: Gluld1, Lgs, Lgsn
Sequence domains: Glutamine synthetase, catalytic domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 17Å
Relevant EMDB volumes: EMD-1290
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Evolution of crystallins for a role in the vertebrate eye lens.
Slingsby et al. (2013)
3 more

1 mention without citation

Data-driven coarse graining of large biomolecular structures.
Chen et al. (2017)