› 2jdh

X-ray diffraction
1.1Å resolution

Lectin PA-IIL of P.aeruginosa complexed with disaccharide derivative

Released:
DOI: 10.2210/pdb2jdh/pdb
PDB entry 2jdh coloured by chain, front view.
PDB entry 2jdh coloured by chain, side view.
PDB entry 2jdh coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
X-ray structures and thermodynamics of the interaction of PA-IIL from Pseudomonas aeruginosa with disaccharide derivatives.
Marotte K, Sabin C, Préville C, Moumé-Pymbock M, Wimmerová M, Mitchell EP, Imberty A, Roy R
ChemMedChem 2 1328-38 (2007)
PMID: 17623286

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Calcium-mediated lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 115 amino acids
Theoretical weight: 11.87 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HYN5 (Residues: 1-115; Coverage: 100%)
Gene names: PA3361, lecB
Sequence domains: Fucose-binding lectin II (PA-IIL)
Structure domains: Calcium-mediated lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: FUC, NAG
Carbohydrate polymer
4 bound ligands:
Ligand FUC 1 x FUC
Ligand CA 8 x CA
Ligand TA5 3 x TA5
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 52.552Å b: 72.829Å c: 54.359Å
α: 90° β: 94.29° γ: 90°
R-values:
R R work R free
0.108 0.107 0.131
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

From carbohydrate leads to glycomimetic drugs.
Ernst et al. (2009)
6 more

2 mentions without citation

Structural basis for norovirus inhibition and fucose mimicry by citrate.
Hansman et al. (2012)
1 more