› 2jfm

X-ray diffraction
2.85Å resolution

CRYSTAL STRUCTURE OF HUMAN STE20-LIKE KINASE (UNLIGANDED FORM)

Released:
DOI: 10.2210/pdb2jfm/pdb
PDB entry 2jfm coloured by chain, front view.
PDB entry 2jfm coloured by chain, side view.
PDB entry 2jfm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites.
Pike AC, Rellos P, Niesen FH, Turnbull A, Oliver AW, Parker SA, Turk BE, Pearl LH, Knapp S
OpenAccess logo EMBO J 27 704-14 (2008)
PMID: 18239682

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
STE20-like serine/threonine-protein kinase Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 37.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H2G2 (Residues: 12-15, 16-320; Coverage: 25%)
Gene names: KIAA0204, SLK, STK2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6122
Unit cell:
a: 102.073Å b: 102.073Å c: 176.993Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.23 0.271
Expression system: Escherichia coli BL21(DE3)

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Citations

17 review citations

Structure and Molecular Mechanism of ER Stress Signaling by the Unfolded Protein Response Signal Activator IRE1.
Adams et al. (2019)
16 more

2 mentions without citation

Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites.
Pike et al. (2008)
1 more