› 2jj4

X-ray diffraction
3.46Å resolution

The complex of PII and acetylglutamate kinase from Synechococcus elongatus PCC7942

Released:
DOI: 10.2210/pdb2jj4/pdb
PDB entry 2jj4 coloured by chain, front view.
PDB entry 2jj4 coloured by chain, side view.
PDB entry 2jj4 coloured by chain, top view.
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Primary publication:
The crystal structure of the complex of PII and acetylglutamate kinase reveals how PII controls the storage of nitrogen as arginine.
Llácer JL, Contreras A, Forchhammer K, Marco-Marín C, Gil-Ortiz F, Maldonado R, Fita I, Rubio V
Proc Natl Acad Sci U S A 104 17644-9 (2007)
PMID: 17959776

Function and Biology Details

Reaction catalysed: 
ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141206 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Acetylglutamate kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 321 amino acids
Theoretical weight: 34.49 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q6V1L5 (Residues: 1-301; Coverage: 100%)
Gene names: Synpcc7942_1496, argB
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like
Nitrogen regulatory protein P-II Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 112 amino acids
Theoretical weight: 12.41 KDa
Source organism: Synechococcus elongatus PCC 7942 = FACHB-805
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A3F4 (Residues: 1-112; Coverage: 100%)
Gene names: Synpcc7942_0321, glnB
Sequence domains: Nitrogen regulatory protein P-II
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand NLG 2 x NLG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2221
Unit cell:
a: 106.901Å b: 149.539Å c: 162.205Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.233 0.294
Expression system: Escherichia coli BL21

Quick links

Citations

13 review citations

P(II) signal transducers: novel functional and structural insights.
Forchhammer K. (2008)
12 more

1 mention without citation

The crystal structure of the complex of PII and acetylglutamate kinase reveals how PII controls the storage of nitrogen as arginine.
Llácer et al. (2007)