› 2jjf

X-ray diffraction
1.95Å resolution

N328A mutant of M. tuberculosis Rv3290c

Released:
DOI: 10.2210/pdb2jjf/pdb
PDB entry 2jjf coloured by chain, front view.
PDB entry 2jjf coloured by chain, side view.
PDB entry 2jjf coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Mutational analysis of Mycobacterium tuberculosis lysine ɛ-aminotransferase and inhibitor co-crystal structures, reveals distinct binding modes.
Tripathi SM, Agarwal A, Ramachandran R
Biochem Biophys Res Commun 463 154-60
PMID: 26003725

Function and Biology Details

Reaction catalysed: 
L-lysine + 2-oxoglutarate = (S)-2-amino-6-oxohexanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable L-lysine-epsilon aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 49.03 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQ77 (Residues: 1-449; Coverage: 100%)
Gene names: MTCY71.30, Rv3290c, lat
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 103.407Å b: 103.407Å c: 98.162Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.166 0.185
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The pathogenic mechanism of Mycobacterium tuberculosis: implication for new drug development.
Yan et al. (2022)

1 mention without citation

phenix.model_vs_data: a high-level tool for the calculation of crystallographic model and data statistics.
Afonine et al. (2010)