› 2jjh

X-ray diffraction
2.7Å resolution

E243 mutant of M. tuberculosis Rv3290C

Released:
DOI: 10.2210/pdb2jjh/pdb
PDB entry 2jjh coloured by chain, front view.
PDB entry 2jjh coloured by chain, side view.
PDB entry 2jjh coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Mutational analysis of Mycobacterium tuberculosis lysine ɛ-aminotransferase and inhibitor co-crystal structures, reveals distinct binding modes.
Tripathi SM, Agarwal A, Ramachandran R
Biochem Biophys Res Commun 463 154-60
PMID: 26003725

Function and Biology Details

Reaction catalysed: 
L-lysine + 2-oxoglutarate = (S)-2-amino-6-oxohexanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable L-lysine-epsilon aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 449 amino acids
Theoretical weight: 49.01 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQ77 (Residues: 1-449; Coverage: 100%)
Gene names: MTCY71.30, Rv3290c, lat
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand AKG 1 x AKG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 102.885Å b: 102.885Å c: 97.816Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.152 0.15 0.194
Expression system: Escherichia coli

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Citations

1 review citation

The pathogenic mechanism of Mycobacterium tuberculosis: implication for new drug development.
Yan et al. (2022)