› 2jpp

Solution NMR

Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA

Released:
DOI: 10.2210/pdb2jpp/pdb
PDB entry 2jpp coloured by chain, ensemble of 10 models, front view.
PDB entry 2jpp coloured by chain, ensemble of 10 models, side view.
PDB entry 2jpp coloured by chain, ensemble of 10 models, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Molecular basis of messenger RNA recognition by the specific bacterial repressing clamp RsmA/CsrA.
Schubert M, Lapouge K, Duss O, Oberstrass FC, Jelesarov I, Haas D, Allain FH
Nat Struct Mol Biol 14 807-13 (2007)
PMID: 17704818

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-534559 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Translational regulator CsrA1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 70 amino acids
Theoretical weight: 7.85 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DPC3 (Residues: 1-64; Coverage: 100%)
Gene names: csrA1, rsmE
Sequence domains: Global regulator protein family
RNA (5'-R(*GP*GP*GP*CP*UP*UP*CP*AP*CP*GP*GP*AP*UP*GP*AP*AP*GP*CP*CP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 20 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Pseudomonas fluorescens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

Bacterial small RNA regulators: versatile roles and rapidly evolving variations.
Gottesman et al. (2011)
24 more

12 mentions without citation

Regulation of bacterial virulence by Csr (Rsm) systems.
Vakulskas et al. (2015)
11 more