› 2kfb

Solution NMR

The structure of the cataract causing P23T mutant of human gamma-D crystallin

Released:
DOI: 10.2210/pdb2kfb/pdb
PDB entry 2kfb coloured by chain, ensemble of 20 models, front view.
PDB entry 2kfb coloured by chain, ensemble of 20 models, side view.
PDB entry 2kfb coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the cataract-causing P23T mutant of human gammaD-crystallin exhibits distinctive local conformational and dynamic changes.
Jung J, Byeon IJ, Wang Y, King J, Gronenborn AM
OpenAccess logo Biochemistry 48 2597-609 (2009)
PMID: 19216553

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-crystallin D Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 21.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07320 (Residues: 2-174; Coverage: 99%)
Gene names: CRYG4, CRYGD
Sequence domains: Beta/Gamma crystallin
Structure domains: Crystallins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics, simulated annealing
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Protein misfolding and aggregation in cataract disease and prospects for prevention.
Moreau et al. (2012)
3 more

4 mentions without citation

Evolution of crystallins for a role in the vertebrate eye lens.
Slingsby et al. (2013)
3 more