› 2kim

Solution NMR

1.7-mm microcryoprobe solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.

Released:
DOI: 10.2210/pdb2kim/pdb
PDB entry 2kim coloured by chain, ensemble of 20 models, front view.
PDB entry 2kim coloured by chain, ensemble of 20 models, side view.
PDB entry 2kim coloured by chain, ensemble of 20 models, top view.
Source organism: Vibrio parahaemolyticus AQ3810
Entry authors: Aramini JM, Belote RL, Ciccosanti CT, Jiang M, Rost B, Nair R, Swapna GVT, Acton TB, Xiao R, Everett JK, Montelione GT, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107830 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA base-flipping protein Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.34 KDa
Source organism: Vibrio parahaemolyticus AQ3810
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6B4U8 (Residues: 1-100; Coverage: 100%)
Gene names: A79_1377, D030_1023
Sequence domains: 6-O-methylguanine DNA methyltransferase, DNA binding domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

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