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Solution structure and functional analysis of the influenza B proton channel.

<div class='caption-title'>Solution structure of BM2 from influenza B virus</div>
<div class='caption-title'>Assembly of the H+ conducting pore</div>
<div class='caption-title'>Interaction between BM2 cytoplasmic domain and BM1 matrix protein</div>
Wang J, Pielak RM, McClintock MA, Chou JJ
OpenAccess logo Nat Struct Mol Biol 16 1267-71 (2009)
Cited: 81 times
EuropePMC logo PMID: 19898475

Abstract

Influenza B virus contains an integral membrane protein, BM2, that oligomerizes in the viral membrane to form a pH-activated proton channel. Here we report the solution structures of both the membrane-embedded channel domain and the cytoplasmic domain of BM2. The channel domain assumes a left-handed coiled-coil tetramer formation with a helical packing angle of -37 degrees to form a polar pore in the membrane for conducting ions. Mutagenesis and proton flux experiments identified residues involved in proton relay and suggest a mechanism of proton conductance. The cytoplasmic domain of BM2 also forms a coiled-coil tetramer. It has a bipolar charge distribution, in which a negatively charged region interacts specifically with the M1 matrix protein that is involved in packaging the genome in the virion. This interaction suggests BM2 also recruits matrix proteins to the cell surface during virus budding, making BM2 an unusual membrane protein with the dual roles of conducting ions and recruiting proteins to the membrane.

Reviews - 2kj1 mentioned but not cited (2)

  1. Influenza M2 proton channels. Pielak RM, Chou JJ. Biochim Biophys Acta 1808 522-529 (2011)
  2. Mechanism of function of viral channel proteins and implications for drug development. Fischer WB, Wang YT, Schindler C, Chen CP. Int Rev Cell Mol Biol 294 259-321 (2012)

Articles - 2kj1 mentioned but not cited (2)



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