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Structural basis for the photoconversion of a phytochrome to the activated Pfr form.

<div class='caption-title'>Three-dimensional overlay of SyB-Cph1(GAF) Pr and Pfr solution structures</div>
<div class='caption-title'>Rotation of the A ring of the PCB chromophore during Pr to Pfr photoconversion</div>
<div class='caption-title'>Light-driven conformational changes for amino acids surrounding the chromophore</div>
Ulijasz AT, Cornilescu G, Cornilescu CC, Zhang J, Rivera M, Markley JL, Vierstra RD
OpenAccess logo Nature 463 250-4 (2010)
Related entries: 2k2n, 2kli, 2lb5, 2lb9

Cited: 66 times
EuropePMC logo PMID: 20075921

Abstract

Phytochromes are a collection of bilin-containing photoreceptors that regulate numerous photoresponses in plants and microorganisms through their ability to photointerconvert between a red-light-absorbing, ground state (Pr) and a far-red-light-absorbing, photoactivated state (Pfr). Although the structures of several phytochromes as Pr have been determined, little is known about the structure of Pfr and how it initiates signalling. Here we describe the three-dimensional solution structure of the bilin-binding domain as Pfr, using the cyanobacterial phytochrome from Synechococcus OSB'. Contrary to predictions, light-induced rotation of the A pyrrole ring but not the D ring is the primary motion of the chromophore during photoconversion. Subsequent rearrangements within the protein then affect intradomain and interdomain contact sites within the phytochrome dimer. On the basis of our models, we propose that phytochromes act by propagating reversible light-driven conformational changes in the bilin to altered contacts between the adjacent output domains, which in most phytochromes direct differential phosphotransfer.

Articles - 2koi mentioned but not cited (2)

  1. Structural basis for the photoconversion of a phytochrome to the activated Pfr form. Ulijasz AT, Cornilescu G, Cornilescu CC, Zhang J, Rivera M, Markley JL, Vierstra RD. Nature 463 250-254 (2010)
  2. The D-ring, not the A-ring, rotates in Synechococcus OS-B' phytochrome. Song C, Psakis G, Kopycki J, Lang C, Matysik J, Hughes J. J. Biol. Chem. 289 2552-2562 (2014)


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Related citations provided by authors (1)

  1. Solution structure of a cyanobacterial phytochrome GAF domain in the red-light-absorbing ground state.. Cornilescu G, Ulijasz AT, Cornilescu CC, Markley JL, Vierstra RD J Mol Biol 383 403-13 (2008)

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