2ky8

Solution NMR

Solution structure and dynamic analysis of chicken MBD2 methyl binding domain bound to a target methylated DNA sequence

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-115508 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
MBD domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 72 amino acids
Theoretical weight: 8.01 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5EFL0 (Residues: 2-71; Coverage: 27%)
Gene names: LOC121108245, MBD2
Sequence domains: Methyl-CpG binding domain
Structure domains: Methyl-cpg-binding Protein 2; Chain A
DNA (5'-D(*GP*GP*AP*AP*TP*(5CM)P*GP*GP*CP*(TED)P*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.76 KDa
Source organism: Gallus gallus
Expression system: Not provided
DNA (5'-D(*GP*AP*GP*CP*(5CM)P*GP*AP*TP*(TED)P*CP*C)-3') Chain: C

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 62%
Refinement method: torsion angle dynamics
Expression systems:
  • Escherichia coli
  • Not provided