› 2kzr

Solution NMR

Solution NMR Structure of Ubiquitin thioesterase OTU1 (EC 3.1.2.-) from Mus musculus, Northeast Structural Genomics Consortium Target MmT2A

Released:
DOI: 10.2210/pdb2kzr/pdb
PDB entry 2kzr coloured by chain, ensemble of 20 models, front view.
PDB entry 2kzr coloured by chain, ensemble of 20 models, side view.
PDB entry 2kzr coloured by chain, ensemble of 20 models, top view.
Source organism: Mus musculus
Entry authors: Chitayat S, Gutmanas A, Lemak A, Yee A, Bezsonova I, Wu B, Doherty RS, Semesi A, Montelione GT, Arrowsmith CH, Dhe-Paganon S, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin thioesterase OTU1 Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8CB27 (Residues: 42-127; Coverage: 25%)
Gene name: Yod1
Sequence domains: OTU1, UBXL domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 89%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links