› 2lqm

Solution NMR

Solution Structures of RadA intein from Pyrococcus horikoshii

Released:
DOI: 10.2210/pdb2lqm/pdb
PDB entry 2lqm coloured by chain, ensemble of 20 models, front view.
PDB entry 2lqm coloured by chain, ensemble of 20 models, side view.
PDB entry 2lqm coloured by chain, ensemble of 20 models, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
NMR and crystal structures of the Pyrococcus horikoshii RadA intein guide a strategy for engineering a highly efficient and promiscuous intein.
Oeemig JS, Zhou D, Kajander T, Wlodawer A, Iwaï H
J Mol Biol 421 85-99 (2012)
PMID: 22560994

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pho RadA intein Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 19.92 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58001 (Residues: 153-326; Coverage: 33%)
Gene names: PH0263, radA
Sequence domains: Intein splicing domain
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 89%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Protein splicing: how inteins escape from precursor proteins.
Mills et al. (2014)
5 more

4 mentions without citation

NMR and crystal structures of the Pyrococcus horikoshii RadA intein guide a strategy for engineering a highly efficient and promiscuous intein.
Oeemig et al. (2012)
3 more