› 2lsp

Solution NMR

solution structures of BRD4 second bromodomain with NF-kB-K310ac peptide

Released:
DOI: 10.2210/pdb2lsp/pdb
PDB entry 2lsp coloured by chain, ensemble of 20 models, front view.
PDB entry 2lsp coloured by chain, ensemble of 20 models, side view.
PDB entry 2lsp coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Down-regulation of NF-κB transcriptional activity in HIV-associated kidney disease by BRD4 inhibition.
Zhang G, Liu R, Zhong Y, Plotnikov AN, Zhang W, Zeng L, Rusinova E, Gerona-Nevarro G, Moshkina N, Joshua J, Chuang PY, Ohlmeyer M, He JC, Zhou MM
J Biol Chem 287 28840-51 (2012)
PMID: 22645123

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-521205 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription factor p65 Chain: A
Molecule details ›
Chain: A
Length: 13 amino acids
Theoretical weight: 1.66 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q04206 (Residues: 304-316; Coverage: 2%)
Gene names: NFKB3, RELA
Bromodomain-containing protein 4 Chain: B
Molecule details ›
Chain: B
Length: 128 amino acids
Theoretical weight: 14.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60885 (Residues: 333-460; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 79%
Refinement method: DGSA-distance geometry simulated annealing, torsion angle dynamics
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)

Quick links

Citations

45 review citations

Targeting bromodomains: epigenetic readers of lysine acetylation.
Filippakopoulos et al. (2014)
44 more

2 mentions without citation

Down-regulation of NF-κB transcriptional activity in HIV-associated kidney disease by BRD4 inhibition.
Zhang et al. (2012)
1 more