› 2m02

Solid-state NMR

3D structure of cap-gly domain of mammalian dynactin determined by magic angle spinning NMR spectroscopy

Released:
DOI: 10.2210/pdb2m02/pdb
PDB entry 2m02 coloured by chain, ensemble of 10 models, front view.
PDB entry 2m02 coloured by chain, ensemble of 10 models, side view.
PDB entry 2m02 coloured by chain, ensemble of 10 models, top view.
Source organism: Rattus norvegicus
Primary publication:
Three-dimensional structure of CAP-gly domain of mammalian dynactin determined by magic angle spinning NMR spectroscopy: conformational plasticity and interactions with end-binding protein EB1.
Yan S, Hou G, Schwieters CD, Ahmed S, Williams JC, Polenova T
J Mol Biol 425 4249-66 (2013)
PMID: 23648839

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151159 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dynactin subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 89 amino acids
Theoretical weight: 9.53 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28023 (Residues: 19-107; Coverage: 7%)
Gene name: Dctn1
Sequence domains: CAP-Gly domain
Structure domains: CAP Gly-rich-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 34%
Refinement method: simulated annealing, torsion angle dynamics, molecular dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Accuracy and precision of protein structures determined by magic angle spinning NMR spectroscopy: for some 'with a little help from a friend'.
Russell et al. (2019)
5 more

7 mentions without citation

Structural biology of supramolecular assemblies by magic-angle spinning NMR spectroscopy.
Quinn et al. (2017)
6 more