› 2m6m

Solution NMR

Solution structure of RING domain of E3 ubiquitin ligase Doa10

Released:
DOI: 10.2210/pdb2m6m/pdb
PDB entry 2m6m coloured by chain, ensemble of 19 models, front view.
PDB entry 2m6m coloured by chain, ensemble of 19 models, side view.
PDB entry 2m6m coloured by chain, ensemble of 19 models, top view.
Source organism: Saccharomyces cerevisiae S288C
Entry authors: Lim J, Son W

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ERAD-associated E3 ubiquitin-protein ligase DOA10 Chain: A
Molecule details ›
Chain: A
Length: 83 amino acids
Theoretical weight: 9.22 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40318 (Residues: 19-101; Coverage: 6%)
Gene names: DOA10, SSM4, YI3299.01C, YI9905.18C, YIL030C
Sequence domains: RING-variant domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: molecular dynamics
Expression system: Escherichia coli

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