› 2m8e

Solution NMR

NMR structure of the PAI subdomain of Sleeping Beauty transposase

Released:
DOI: 10.2210/pdb2m8e/pdb
PDB entry 2m8e coloured by chain, ensemble of 14 models, front view.
PDB entry 2m8e coloured by chain, ensemble of 14 models, side view.
PDB entry 2m8e coloured by chain, ensemble of 14 models, top view.
Source organism: synthetic construct
Primary publication:
NMR structural analysis of Sleeping Beauty transposase binding to DNA.
Carpentier CE, Schreifels JM, Aronovich EL, Carlson DF, Hackett PB, Nesmelova IV
Protein Sci 23 23-33 (2014)
PMID: 24243759

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162983 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SLEEPING BEAUTY TRANSPOSASE Chain: A
Molecule details ›
Chain: A
Length: 57 amino acids
Theoretical weight: 6.39 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 49%
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

4 review citations

DNA Transposition at Work.
Hickman et al. (2016)
3 more

3 mentions without citation

NMR structural analysis of Sleeping Beauty transposase binding to DNA.
Carpentier et al. (2014)
2 more