› 2mlb

Solution NMR

NMR solution structure of a computational designed protein based on template of human erythrocytic ubiquitin

Released:
DOI: 10.2210/pdb2mlb/pdb
PDB entry 2mlb coloured by chain, ensemble of 20 models, front view.
PDB entry 2mlb coloured by chain, ensemble of 20 models, side view.
PDB entry 2mlb coloured by chain, ensemble of 20 models, top view.
Source organism: synthetic construct
Primary publication:
Protein design with a comprehensive statistical energy function and boosted by experimental selection for foldability.
Xiong P, Wang M, Zhou X, Zhang T, Zhang J, Chen Q, Liu H
Nat Commun 5 5330 (2014)
PMID: 25345468

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163004 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
redesigned ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 79 amino acids
Theoretical weight: 8.56 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 85%
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

4 review citations

Third generation antibody discovery methods: in silico rational design.
Sormanni et al. (2018)
3 more

1 mention without citation

Multiple GPCR Functional Assays Based on Resonance Energy Transfer Sensors.
Zhou et al. (2021)