› 2mow

Solution NMR

Structure of Nrd1p CID - Trf4p NIM complex

Released:
DOI: 10.2210/pdb2mow/pdb
PDB entry 2mow coloured by chain, ensemble of 20 models, front view.
PDB entry 2mow coloured by chain, ensemble of 20 models, side view.
PDB entry 2mow coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Molecular basis for coordinating transcription termination with noncoding RNA degradation.
Tudek A, Porrua O, Kabzinski T, Lidschreiber M, Kubicek K, Fortova A, Lacroute F, Vanacova S, Cramer P, Stefl R, Libri D
OpenAccess logo Mol Cell 55 467-81 (2014)
PMID: 25066235

Function and Biology Details

Reaction catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156832 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein NRD1 Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 18.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P53617 (Residues: 1-153; Coverage: 27%)
Gene names: N0868, NRD1, YNL251C
Sequence domains: CID domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Poly(A) RNA polymerase protein 2 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P53632 (Residues: 573-584; Coverage: 2%)
Gene names: HRC584, O0716, PAP2, TRF4, YOL115W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 71%
Refinement method: simulated annealing
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided

Quick links

Citations

13 review citations

The regulation and functions of the nuclear RNA exosome complex.
Kilchert et al. (2016)
12 more

1 mention without citation

Exosome Cofactors Connect Transcription Termination to RNA Processing by Guiding Terminated Transcripts to the Appropriate Exonuclease within the Nuclear Exosome.
Kim et al. (2016)