› 2mpk

Solution NMR

Characterization and structure of the MIT1 domain of a chitin synthase from the Oomycete Saprolegnia monoica

Released:
DOI: 10.2210/pdb2mpk/pdb
PDB entry 2mpk coloured by chain, ensemble of 20 models, front view.
PDB entry 2mpk coloured by chain, ensemble of 20 models, side view.
PDB entry 2mpk coloured by chain, ensemble of 20 models, top view.
Source organism: Saprolegnia monoica
Primary publication:
Structural and functional characterization of the microtubule interacting and trafficking domains of two oomycete chitin synthases.
Brown C, Szpryngiel S, Kuang G, Srivastava V, Ye W, McKee LS, Tu Y, Mäler L, Bulone V
FEBS J 283 3072-88 (2016)
PMID: 27363606

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
chitin synthase Chain: A
Molecule details ›
Chain: A
Length: 74 amino acids
Theoretical weight: 8.53 KDa
Source organism: Saprolegnia monoica
Expression system: Escherichia coli
UniProt:
  • Canonical: D7NQS8 (Residues: 103-171; Coverage: 8%)
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this NMR entry.
Chemical shift assignment: 82%
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

1 citations in other articles

Identification and Characterization of the Chitin Synthase Genes From the Fish Pathogen Saprolegnia parasitica.
Rzeszutek et al. (2019)

3 mentions without citation

Structural basis for directional chitin biosynthesis.
Chen et al. (2022)
2 more