› 2msj

X-ray diffraction
1.9Å resolution

TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 N46S

Released:
DOI: 10.2210/pdb2msj/pdb
PDB entry 2msj coloured by chain, front view.
PDB entry 2msj coloured by chain, side view.
PDB entry 2msj coloured by chain, top view.
Source organism: Zoarces americanus
Primary publication:
Quantitative and qualitative analysis of type III antifreeze protein structure and function.
Graether SP, DeLuca CI, Baardsnes J, Hill GA, Davies PL, Jia Z
J Biol Chem 274 11842-7 (1999)
PMID: 10207002

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148668 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-3 ice-structuring protein HPLC 12 Chain: A
Molecule details ›
Chain: A
Length: 66 amino acids
Theoretical weight: 6.95 KDa
Source organism: Zoarces americanus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19614 (Residues: 1-65; Coverage: 99%)
Structure domains: Antifreeze-like/N-acetylneuraminic acid synthase C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 32.761Å b: 38.997Å c: 46.951Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.242 0.326
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Nanoparticles, Proteins, and Nucleic Acids: Biotechnology Meets Materials Science.
Niemeyer CM. (2001)
1 more

3 mentions without citation

Identification of antifreeze proteins and their functional residues by support vector machine and genetic algorithms based on n-peptide compositions.
Yu et al. (2011)
2 more