2n2r › Citations

The radish defensins RsAFP1 and RsAFP2 act synergistically with caspofungin against Candida albicans biofilms.

Vriens K, Cools TL, Harvey PJ, Craik DJ, Braem A, Vleugels J, De Coninck B, Cammue BP, Thevissen K
Peptides 75 71-9 (2016)
Cited: 38 times
EuropePMC logo PMID: 26592804

Abstract

The radish defensin RsAFP2 was previously characterized as a peptide with potent antifungal activity against several plant pathogenic fungi and human pathogens, including Candida albicans. RsAFP2 induces apoptosis and impairs the yeast-to-hypha transition in C. albicans. As the yeast-to-hypha transition is considered important for progression to mature biofilms, we analyzed the potential antibiofilm activity of recombinant (r)RsAFP2, heterologously expressed in Pichia pastoris, against C. albicans biofilms. We found that rRsAFP2 prevents C. albicans biofilm formation with a BIC-2 (i.e., the minimal rRsAFP2 concentration that inhibits biofilm formation by 50% as compared to control treatment) of 1.65 ± 0.40 mg/mL. Moreover, biofilm-specific synergistic effects were observed between rRsAFP2 doses as low as 2.5 μg/mL to 10 μg/mL and the antimycotics caspofungin and amphotericin B, pointing to the potential of RsAFP2 as a novel antibiofilm compound. In addition, we characterized the solution structure of rRsAFP2 and compared it to that of RsAFP1, another defensin present in radish seeds. These peptides have similar amino acid sequences, except for two amino acids, but rRsAFP2 is more potent than RsAFP1 against planktonic and biofilm cultures. Interestingly, as in case of rRsAFP2, also RsAFP1 acts synergistically with caspofungin against C. albicans biofilms in a comparable low dose range as rRsAFP2. A structural comparison of both defensins via NMR analysis revealed that also rRsAFP2 adopts the typical cysteine-stabilized αβ-motif of plant defensins, however, no structural differences were found between these peptides that might result in their differential antifungal/antibiofilm potency. This further suggests that the conserved structure of RsAFP1 and rRsAFP2 bears the potential to synergize with antimycotics against C. albicans biofilms.

Reviews - 2n2r mentioned but not cited (2)

  1. Plant Defensins from a Structural Perspective. Kovaleva V, Bukhteeva I, Kit OY, Nesmelova IV. Int J Mol Sci 21 E5307 (2020)
  2. Short Peptides Make a Big Difference: The Role of Botany-Derived AMPs in Disease Control and Protection of Human Health. Luo X, Wu W, Feng L, Treves H, Ren M. Int J Mol Sci 22 11363 (2021)

Articles - 2n2r mentioned but not cited (2)

  1. Structural and biological features of a novel plant defensin from Brugmansia x candida. Kaewklom S, Wongchai M, Petvises S, Hanpithakphong W, Aunpad R. PLoS One 13 e0201668 (2018)
  2. Analysis of mutations of defensin protein using accelerated molecular dynamics simulations. Pandey B, Tyagi C, Prajapati GK, Mishra AK, Hashem A, Alqarawi AA, Abd Allah EF, Mohanta TK. PLoS One 15 e0241679 (2020)


Reviews citing this publication (16)

  1. Antifungal Peptides as Therapeutic Agents. Fernández de Ullivarri M, Arbulu S, Garcia-Gutierrez E, Cotter PD. Front Cell Infect Microbiol 10 105 (2020)
  2. Natural Antimicrobial Peptides as Inspiration for Design of a New Generation Antifungal Compounds. Bondaryk M, Staniszewska M, Zielińska P, Urbańczyk-Lipkowska Z. J Fungi (Basel) 3 E46 (2017)
  3. Methodologies for in vitro and in vivo evaluation of efficacy of antifungal and antibiofilm agents and surface coatings against fungal biofilms. Van Dijck P, Sjollema J, Cammue BP, Lagrou K, Berman J, d'Enfert C, Andes DR, Arendrup MC, Brakhage AA, Calderone R, Cantón E, Coenye T, Cos P, Cowen LE, Edgerton M, Espinel-Ingroff A, Filler SG, Ghannoum M, Gow NAR, Haas H, Jabra-Rizk MA, Johnson EM, Lockhart SR, Lopez-Ribot JL, Maertens J, Munro CA, Nett JE, Nobile CJ, Pfaller MA, Ramage G, Sanglard D, Sanguinetti M, Spriet I, Verweij PE, Warris A, Wauters J, Yeaman MR, Zaat SAJ, Thevissen K. Microb Cell 5 300-326 (2018)
  4. Membrane-Interacting Antifungal Peptides. Struyfs C, Cammue BPA, Thevissen K. Front Cell Dev Biol 9 649875 (2021)
  5. Bioactive Peptides Against Fungal Biofilms. Oshiro KGN, Rodrigues G, Monges BED, Cardoso MH, Franco OL. Front Microbiol 10 2169 (2019)
  6. Antimicrobial Peptides with Anti-Candida Activity. Perez-Rodriguez A, Eraso E, Quindós G, Mateo E. Int J Mol Sci 23 9264 (2022)
  7. Two small, cysteine-rich and cationic antifungal proteins from Penicillium chrysogenum: A comparative study of PAF and PAFB. Huber A, Galgóczy L, Váradi G, Holzknecht J, Kakar A, Malanovic N, Leber R, Koch J, Keller MA, Batta G, Tóth GK, Marx F. Biochim Biophys Acta Biomembr 1862 183246 (2020)
  8. Amino acid-derived defense metabolites from plants: A potential source to facilitate novel antimicrobial development. Parthasarathy A, Borrego EJ, Savka MA, Dobson RCJ, Hudson AO. J Biol Chem 296 100438 (2021)
  9. Plant-Derived Substances in the Fight Against Infections Caused by Candida Species. Guevara-Lora I, Bras G, Karkowska-Kuleta J, González-González M, Ceballos K, Sidlo W, Rapala-Kozik M. Int J Mol Sci 21 E6131 (2020)
  10. Regulated Cell Death as a Therapeutic Target for Novel Antifungal Peptides and Biologics. Yeaman MR, Büttner S, Thevissen K. Oxid Med Cell Longev 2018 5473817 (2018)
  11. The γ-Core Motif Peptides of Plant AMPs as Novel Antimicrobials for Medicine and Agriculture. Slezina MP, Istomina EA, Korostyleva TV, Odintsova TI. Int J Mol Sci 24 483 (2022)
  12. Antimicrobial Peptides: Avant-Garde Antifungal Agents to Fight against Medically Important Candida Species. Rodríguez-Castaño GP, Rosenau F, Ständker L, Firacative C. Pharmaceutics 15 789 (2023)
  13. Defensin-lipid interactions in membrane targeting: mechanisms of action and opportunities for the development of antimicrobial and anticancer therapeutics. Hein MJA, Kvansakul M, Lay FT, Phan TK, Hulett MD. Biochem Soc Trans 50 423-437 (2022)
  14. Exploring the frontiers of therapeutic breadth of antifungal peptides: A new avenue in antifungal drugs. Ul Haq I, Maryam S, Shyntum DY, Khan TA, Li F. J Ind Microbiol Biotechnol 51 kuae018 (2024)
  15. Fighting pathogenic yeasts with plant defensins and anti-fungal proteins from fungi. Manzanares P, Giner-Llorca M, Marcos JF, Garrigues S. Appl Microbiol Biotechnol 108 277 (2024)
  16. Antifungal Plant Defensins as an Alternative Tool to Combat Candidiasis. Finkina EI, Shevchenko OV, Fateeva SI, Tagaev AA, Ovchinnikova TV. Plants (Basel) 13 1499 (2024)

Articles citing this publication (18)

  1. Antifungal plant defensins: increased insight in their mode of action as a basis for their use to combat fungal infections. Cools TL, Struyfs C, Cammue BP, Thevissen K. Future Microbiol 12 441-454 (2017)
  2. Genomic Analysis of Factors Associated with Low Prevalence of Antibiotic Resistance in Extraintestinal Pathogenic Escherichia coli Sequence Type 95 Strains. Stephens CM, Adams-Sapper S, Sekhon M, Johnson JR, Riley LW. mSphere 2 e00390-16 (2017)
  3. A Linear 19-Mer Plant Defensin-Derived Peptide Acts Synergistically with Caspofungin against Candida albicans Biofilms. Cools TL, Struyfs C, Drijfhout JW, Kucharíková S, Lobo Romero C, Van Dijck P, Ramada MHS, Bloch C, Cammue BPA, Thevissen K. Front Microbiol 8 2051 (2017)
  4. Mapping and Identification of Antifungal Peptides in the Putative Antifungal Protein AfpB from the Filamentous Fungus Penicillium digitatum. Garrigues S, Gandía M, Borics A, Marx F, Manzanares P, Marcos JF. Front Microbiol 8 592 (2017)
  5. Hevein-Like Antimicrobial Peptides Wamps: Structure-Function Relationship in Antifungal Activity and Sensitization of Plant Pathogenic Fungi to Tebuconazole by WAMP-2-Derived Peptides. Odintsova T, Shcherbakova L, Slezina M, Pasechnik T, Kartabaeva B, Istomina E, Dzhavakhiya V. Int J Mol Sci 21 E7912 (2020)
  6. The synthetic killer peptide KP impairs Candida albicans biofilm in vitro. Paulone S, Ardizzoni A, Tavanti A, Piccinelli S, Rizzato C, Lupetti A, Colombari B, Pericolini E, Polonelli L, Magliani W, Conti S, Posteraro B, Cermelli C, Blasi E, Peppoloni S. PLoS One 12 e0181278 (2017)
  7. The interaction with fungal cell wall polysaccharides determines the salt tolerance of antifungal plant defensins. Bleackley MR, Dawson CS, Payne JAE, Harvey PJ, Rosengren KJ, Quimbar P, Garcia-Ceron D, Lowe R, Bulone V, van der Weerden NL, Craik DJ, Anderson MA. Cell Surf 5 100026 (2019)
  8. Fungal Glucosylceramide-Specific Camelid Single Domain Antibodies Are Characterized by Broad Spectrum Antifungal Activity. De Coninck B, Verheesen P, Vos CM, Van Daele I, De Bolle MF, Vieira JV, Peferoen M, Cammue BPA, Thevissen K. Front Microbiol 8 1059 (2017)
  9. Synergistic Activity between Two Antifungal Proteins, the Plant Defensin NaD1 and the Bovine Pancreatic Trypsin Inhibitor. Bleackley MR, Dawson CS, McKenna JA, Quimbar P, Hayes BME, van der Weerden NL, Anderson MA. mSphere 2 e00390-17 (2017)
  10. Resistance to the Plant Defensin NaD1 Features Modifications to the Cell Wall and Osmo-Regulation Pathways of Yeast. McColl AI, Bleackley MR, Anderson MA, Lowe RGT. Front Microbiol 9 1648 (2018)
  11. Antifungal Activity, Structural Stability, and Immunomodulatory Effects on Human Immune Cells of Defensin from the Lentil Lens culinaris. Finkina EI, Bogdanov IV, Ignatova AA, Kanushkina MD, Egorova EA, Voropaev AD, Stukacheva EA, Ovchinnikova TV. Membranes (Basel) 12 855 (2022)
  12. Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus. Bard GCV, Taveira GB, Souza TAM, Mello ÉO, Souza SB, Ramos AC, Carvalho AO, Pereira LS, Zottich U, Rodrigues R, Gomes VM. Int J Microbiol 2018 8546470 (2018)
  13. Blad-Containing Oligomer Fungicidal Activity on Human Pathogenic Yeasts. From the Outside to the Inside of the Target Cell. Pinheiro AM, Carreira A, Rollo F, Fernandes R, Ferreira RB, Monteiro SA. Front Microbiol 7 1803 (2016)
  14. Fragments of a Wheat Hevein-Like Antimicrobial Peptide Augment the Inhibitory Effect of a Triazole Fungicide on Spore Germination of Fusarium oxysporum and Alternaria solani. Shcherbakova L, Odintsova T, Pasechnik T, Arslanova L, Smetanina T, Kartashov M, Slezina M, Dzhavakhiya V. Antibiotics (Basel) 9 E870 (2020)
  15. The In Vitro Potential of 1-(1H-indol-3-yl) Derivatives against Candida spp. and Aspergillus niger as Tyrosinase Inhibitors. Gervasi T, Ginestra G, Mancuso F, Barreca D, De Luca L, Mandalari G. Microorganisms 9 2070 (2021)
  16. Bioinformatic analysis of wheat defensin gene family and function verification of candidate genes. Dong Y, Wang Y, Tang M, Chen W, Chai Y, Wang W. Front Plant Sci 14 1279502 (2023)
  17. Characterization of Aspergillus nidulans Biofilm Formation and Structure and Their Inhibition by Pea Defensin Psd2. Corrêa-Almeida C, Borba-Santos LP, Rollin-Pinheiro R, Barreto-Bergter E, Rozental S, Kurtenbach E. Front Mol Biosci 9 795255 (2022)
  18. Plant Defensin PgD1 a Biotechnological Alternative Against Plant Pathogens. de Bem Matos AC, Telli EMRP, Camillo LC, da Silva GF, Gonçalves MJ, Casa R, Rufato L, de Lourdes Borba Magalhães M. Probiotics Antimicrob Proteins (2024)


Quick links