› 2n3g

Solution NMR

Solution structure of DRB4 dsRBD1 (viz. DRB4(1-72))

Released:
DOI: 10.2210/pdb2n3g/pdb
PDB entry 2n3g coloured by chain, ensemble of 20 models, front view.
PDB entry 2n3g coloured by chain, ensemble of 20 models, side view.
PDB entry 2n3g coloured by chain, ensemble of 20 models, top view.
Source organism: Arabidopsis thaliana
Primary publication:
DRB4 dsRBD1 drives dsRNA recognition in Arabidopsis thaliana tasi/siRNA pathway.
Chiliveri SC, Aute R, Rai U, Deshmukh MV
OpenAccess logo Nucleic Acids Res 45 8551-8563 (2017)
PMID: 28575480

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Double-stranded RNA-binding protein 4 Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 16.62 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8H1D4 (Residues: 1-153; Coverage: 43%)
Gene names: At3g62800, DBR4, F26K9.230
Sequence domains: Double-stranded RNA binding motif
Structure domains: Double Stranded RNA Binding Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 83%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

2 review citations

In Vitro Analytical Approaches to Study Plant Ligand-Receptor Interactions.
Sandoval et al. (2020)
1 more

1 mention without citation

DRB4 dsRBD1 drives dsRNA recognition in Arabidopsis thaliana tasi/siRNA pathway.
Chiliveri et al. (2017)