› 2n66

Solution NMR

NMR Structure of sweeter mutant (D40K) of sweet protein Brazzein,

Released:
DOI: 10.2210/pdb2n66/pdb
PDB entry 2n66 coloured by chain, ensemble of 20 models, front view.
PDB entry 2n66 coloured by chain, ensemble of 20 models, side view.
PDB entry 2n66 coloured by chain, ensemble of 20 models, top view.
Source organism: Pentadiplandra brazzeana
Entry authors: Singarapu KK, Tonelli M, Markley JL, Assadi-Porter F

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Defensin-like protein Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 6.52 KDa
Source organism: Pentadiplandra brazzeana
Expression system: Escherichia coli
UniProt:
  • Canonical: P56552 (Residues: 1-54; Coverage: 100%)
Structure domains: Knottin, scorpion toxin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 80%
Refinement method: distance geometry
Expression system: Escherichia coli

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