› 2n8t

Solution NMR

Solution Structure of the rNedd4 WW2 Domain-Cx43CT Peptide Complex by NMR

Released:
DOI: 10.2210/pdb2n8t/pdb
PDB entry 2n8t coloured by chain, ensemble of 10 models, front view.
PDB entry 2n8t coloured by chain, ensemble of 10 models, side view.
PDB entry 2n8t coloured by chain, ensemble of 10 models, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural Studies of the Nedd4 WW Domains and Their Selectivity for the Connexin43 (Cx43) Carboxyl Terminus.
Spagnol G, Kieken F, Kopanic JL, Li H, Zach S, Stauch KL, Grosely R, Sorgen PL
J Biol Chem 291 7637-50 (2016)
PMID: 26841867

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139902 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase NEDD4 Chain: A
Molecule details ›
Chain: A
Length: 39 amino acids
Theoretical weight: 4.44 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q62940 (Residues: 401-437; Coverage: 4%)
Gene names: Nedd4, Nedd4a, Rpf1
Sequence domains: WW domain
Gap junction alpha-1 protein Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.62 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P08050 (Residues: 276-289; Coverage: 4%)
Gene names: Cxn-43, Gja1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: DGSA-distance geometry simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

Role of connexin 43 in different forms of intercellular communication - gap junctions, extracellular vesicles and tunnelling nanotubes.
Ribeiro-Rodrigues et al. (2017)
12 more

2 mentions without citation

Exploring the Roles of HERC2 and the NEDD4L HECT E3 Ubiquitin Ligase Subfamily in p53 Signaling and the DNA Damage Response.
Mathieu et al. (2021)
1 more