› 2nbe

Solution NMR

Solution structure of V26A mutant of Ubiquitin at pH 2.0

Released:
DOI: 10.2210/pdb2nbe/pdb
PDB entry 2nbe coloured by chain, ensemble of 20 models, front view.
PDB entry 2nbe coloured by chain, ensemble of 20 models, side view.
PDB entry 2nbe coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Observing a late folding intermediate of Ubiquitin at atomic resolution by NMR.
Surana P, Das R
Protein Sci 25 1438-50 (2016)
PMID: 27111887

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62987 (Residues: 1-76; Coverage: 59%)
Gene names: UBA52, UBCEP2
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 73%
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

1 review citation

Almost 50 Years of Monomeric Extracellular Ubiquitin (eUb).
Mendoza-Salazar et al. (2024)
3 other articles

1 mention without citation

Observing a late folding intermediate of Ubiquitin at atomic resolution by NMR.
Surana et al. (2016)