2nce

Solution NMR

Solution Structure of Ca2+-bound C2 domain from Protein Kinase C alpha in the form of complex with V5-pHM peptide

Released:
Source organism: Rattus norvegicus
Entry authors: Yang Y, Igumenova TI

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-138745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase C alpha type Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 16.24 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05696 (Residues: 155-293; Coverage: 21%)
Gene names: Pkca, Prkca
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 89%
Refinement method: DGSA-distance geometry simulated annealing
Expression system: Escherichia coli