› 2nco

Solution NMR

NMR solution structure for the C-terminal domain of Tetrahymena Tcb2 in the absence of calcium

Released:
DOI: 10.2210/pdb2nco/pdb
PDB entry 2nco coloured by chain, ensemble of 20 models, front view.
PDB entry 2nco coloured by chain, ensemble of 20 models, side view.
PDB entry 2nco coloured by chain, ensemble of 20 models, top view.
Source organism: Tetrahymena thermophila
Primary publication:
Solution NMR structures of the C-domain of Tetrahymena cytoskeletal protein Tcb2 reveal distinct calcium-induced structural rearrangements.
Kilpatrick AM, Honts JE, Sleister HM, Fowler CA
Proteins 84 1748-1756 (2016)
PMID: 27488393

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140599 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
10 kDa calcium-binding protein Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 11.58 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09226 (Residues: 117-218; Coverage: 47%)
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 89%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

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