› 2no7

X-ray diffraction
1.7Å resolution

C4S dCK variant of dCK in complex with L-dC+ADP

Released:
DOI: 10.2210/pdb2no7/pdb
PDB entry 2no7 coloured by chain, front view.
PDB entry 2no7 coloured by chain, side view.
PDB entry 2no7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Nonenantioselectivity property of human deoxycytidine kinase explained by structures of the enzyme in complex with L- and D-nucleosides.
Sabini E, Hazra S, Konrad M, Lavie A
J Med Chem 50 3004-14 (2007)
PMID: 17530837

Function and Biology Details

Reactions catalysed: 
ATP + deoxyguanosine = ADP + dGMP
NTP + deoxycytidine = NDP + dCMP
ATP + deoxyadenosine = ADP + dAMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-541552 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxycytidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 32.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27707 (Residues: 1-260; Coverage: 100%)
Gene name: DCK
Sequence domains: Deoxynucleoside kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand LDC 2 x LDC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2221
Unit cell:
a: 52.8Å b: 133.35Å c: 156.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.19 0.243
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure, physiological role, and specific inhibitors of human thymidine kinase 2 (TK2): present and future.
Pérez-Pérez et al. (2008)
1 more

2 mentions without citation

Nonenantioselectivity property of human deoxycytidine kinase explained by structures of the enzyme in complex with L- and D-nucleosides.
Sabini et al. (2007)
1 more