› 2np1

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF NITROPHORIN 1 FROM RHODNIUS PROLIXUS

Released:
DOI: 10.2210/pdb2np1/pdb
PDB entry 2np1 coloured by chain, front view.
PDB entry 2np1 coloured by chain, side view.
PDB entry 2np1 coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Crystal structures of a nitric oxide transport protein from a blood-sucking insect.
Weichsel A, Andersen JF, Champagne DE, Walker FA, Montfort WR
Nat Struct Biol 5 304-9 (1998)
PMID: 9546222

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 20.51 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q26239 (Residues: 24-207; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments

3 bound ligands:
Ligand NH4 2 x NH4
Ligand HEM 2 x HEM
Ligand 2HP 2 x 2HP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P21
Unit cell:
a: 39.52Å b: 74.28Å c: 66.41Å
α: 90° β: 99.46° γ: 90°
R-values:
R R work R free
0.208 0.208 0.302
Expression system: Escherichia coli BL21(DE3)

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Citations

18 review citations

Dual action of neutrophil gelatinase-associated lipocalin.
Schmidt-Ott et al. (2007)
17 more

5 mentions without citation

Assignment of the ferriheme resonances of the low-spin complexes of nitrophorins 1 and 4 by (1)H and (13)C NMR spectroscopy: comparison to structural data obtained from X-ray crystallography.
Shokhireva et al. (2007)
4 more