› 2nve

X-ray diffraction
1.5Å resolution

Soluble domain of Rieske Iron Sulfur Protein

Released:
DOI: 10.2210/pdb2nve/pdb
PDB entry 2nve coloured by chain, front view.
PDB entry 2nve coloured by chain, side view.
PDB entry 2nve coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Atomic resolution structures of rieske iron-sulfur protein: role of hydrogen bonds in tuning the redox potential of iron-sulfur clusters.
Kolling DJ, Brunzelle JS, Lhee S, Crofts AR, Nair SK
Structure 15 29-38 (2007)
PMID: 17223530

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 15.18 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02762 (Residues: 47-187; Coverage: 75%)
Gene names: fbcF, petA
Sequence domains: Rieske [2Fe-2S] domain
Structure domains: Rieske [2Fe-2S] iron-sulphur domain

Ligands and Environments

2 bound ligands:
Ligand FES 1 x FES
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: I4
Unit cell:
a: 70.648Å b: 70.648Å c: 54.717Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.133 0.174
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
7 more

3 mentions without citation

The Q-cycle reviewed: How well does a monomeric mechanism of the bc(1) complex account for the function of a dimeric complex?
Crofts et al. (2008)
2 more