› 2o0c

X-ray diffraction
2.6Å resolution

Crystal structure of the H-NOX domain from Nostoc sp. PCC 7120 complexed to NO

Released:
DOI: 10.2210/pdb2o0c/pdb
PDB entry 2o0c coloured by chain, front view.
PDB entry 2o0c coloured by chain, side view.
PDB entry 2o0c coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Primary publication:
NO and CO differentially activate soluble guanylyl cyclase via a heme pivot-bend mechanism.
Ma X, Sayed N, Beuve A, van den Akker F
EMBO J 26 578-88 (2007)
PMID: 17215864

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-571058 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme NO-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 21.21 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8YUQ7 (Residues: 1-189; Coverage: 100%)
Gene name: alr2278
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

2 bound ligands:
Ligand HEM 2 x HEM
Ligand NO 2 x NO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P213
Unit cell:
a: 123.564Å b: 123.564Å c: 123.564Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.192 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

Concepts of neural nitric oxide-mediated transmission.
Garthwaite J. (2008)
29 more

6 mentions without citation

Carbon monoxide--physiology, detection and controlled release.
Heinemann et al. (2014)
5 more