› 2o0z

X-ray diffraction
2Å resolution

Mycobacterium tuberculosis epsp synthase in complex with product (EPS)

Released:
DOI: 10.2210/pdb2o0z/pdb
PDB entry 2o0z coloured by chain, front view.
PDB entry 2o0z coloured by chain, side view.
PDB entry 2o0z coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Kachalova GS, Bartunik HD, Mycobacterium Tuberculosis Structural Proteomics Project (XMTB)

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162027 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phosphoshikimate 1-carboxyvinyltransferase Chain: A
Molecule details ›
Chain: A
Length: 450 amino acids
Theoretical weight: 46.47 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPY5 (Residues: 1-450; Coverage: 100%)
Gene names: Rv3227, aroA
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand PO4 1 x PO4
Ligand EPS 1 x EPS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 40.729Å b: 73.104Å c: 128.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.153 not available
Expression system: Escherichia coli

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